FastQCFastQC Report
Mon 6 Feb 2017
SRR2077225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4900559
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51050.036.3139151
GTATCAA101700.034.123241
ATCAACG129600.026.1353
TATCAAC131200.026.0783752
TCAACGC129800.025.8295484
CAACGCA133200.025.2218655
AACGCAG138200.024.11036
ACGCAGA157700.020.7851527
CGCAGAG159850.020.1955438
GAGTACT122250.019.43005812
AGAGTAC156900.019.41134811
TAGCCCT31950.018.83454
CAGAGTA166300.018.5627510
GTACATG96050.018.4770371
AGTACTT130200.018.34908113
GTACTTT133750.017.97293714
GCAGAGT175300.017.7675139
TACATGG94900.017.5013772
ACATGGG100300.015.7705993
GTGTAGC25600.015.7190361
Produced by FastQC (version 0.11.5)