FastQCFastQC Report
Mon 6 Feb 2017
SRR2077224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4486620
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63800.030.03371
GGTATCA35650.026.5844631
TCAACGC73950.024.6969494
ATCAACG74850.024.5872423
TATCAAC78350.023.7988832
CAACGCA77250.023.7313185
AACGCAG80450.022.4425586
GTACATG99150.019.6733551
TACATGG95600.019.1441022
ACGCAGA93700.019.0481387
CGCAGAG94300.018.7081228
CGATACG950.007789464718.1774367
AGAGTAC90750.017.74464611
ACATGGG100550.017.5474193
TAGCCCT27050.017.2302824
GAGTACT49800.017.03402912
CAGAGTA103050.015.8849510
CATGGGG64250.015.0993784
GCAGAGT111450.014.8691819
GTATAGC14950.014.75344851
Produced by FastQC (version 0.11.5)