FastQCFastQC Report
Mon 6 Feb 2017
SRR2077224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4486620
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.036.0745351
GTATCAA100150.034.0041431
ATCAACG127300.026.0949063
TCAACGC127000.025.9940874
TATCAAC130050.025.5959952
CAACGCA129850.025.3176275
AACGCAG134400.024.4605186
ACGCAGA154900.021.006657
CGCAGAG155800.020.7229658
AGAGTAC152350.020.06043411
GTACATG96650.019.7888471
GAGTACT117400.019.03383412
CAGAGTA164450.018.83653510
TACATGG96850.018.2858282
GCAGAGT172350.018.1734169
AGTACTT126250.017.86256413
GTACTTT129950.017.57325214
ACATGGG99500.017.1421623
ACCGTGC6200.016.082748
TACTTTT148600.015.30879615
Produced by FastQC (version 0.11.5)