FastQCFastQC Report
Mon 6 Feb 2017
SRR2077223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4113511
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85300.028.6491371
GGTATCA47250.023.6351051
TCAACGC101200.023.1949464
ATCAACG101600.023.1058833
CAACGCA104300.022.604565
TATCAAC107000.022.226672
AACGCAG107500.021.8655956
GTACATG120550.020.271851
AGAGTAC104700.020.08718711
TACATGG121900.019.3402232
ACGCAGA122350.019.0428967
CGCAGAG123250.018.8205138
GAGTACT53850.018.05463812
ACATGGG127000.018.049773
CAGAGTA128500.016.8144910
GCAGAGT132350.016.5353899
GTATACG2151.2895998E-616.0315651
GAGTACA89150.016.006391
AGTACTT62800.015.42891913
CATGGGA56200.015.3556674
Produced by FastQC (version 0.11.5)