FastQCFastQC Report
Mon 6 Feb 2017
SRR2077223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4113511
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.11192385288382602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131900.033.3245851
GGTATCA67300.031.8895361
ATCAACG173700.025.024033
TATCAAC173050.024.9990962
TCAACGC173850.024.7452934
CAACGCA177600.024.2225065
AACGCAG183900.023.1870046
GAGTACT139500.020.84795212
AGAGTAC190100.020.56106611
ACGCAGA207250.020.2760147
AGTACTT146150.020.06375513
GTACATG118600.020.0388161
CGCAGAG207500.019.9824778
GTACTTT152000.019.30072214
TACATGG120100.019.2129822
CAGAGTA216500.018.35688210
GCAGAGT221050.018.1661079
ACATGGG126750.017.7163893
TCGTATG4950.017.58547640
TACTTTT169800.017.36455515
Produced by FastQC (version 0.11.5)