FastQCFastQC Report
Mon 6 Feb 2017
SRR2077222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909852
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62650.032.4554821
GGTATCA33100.029.4656221
TCAACGC74650.026.1681084
ATCAACG75700.026.125633
CAACGCA75900.025.6462025
TATCAAC79800.025.0491712
AACGCAG78900.024.4954626
ACGCAGA93900.020.3621837
GTACATG112850.020.0641571
CGCAGAG95050.019.8253428
TACATGG111250.019.6097452
AGAGTAC93000.019.12966511
ACATGGG115700.018.3165233
CAGAGTA101900.017.99147610
GCAGAGT105500.017.437899
GTGTAGC19950.017.2748471
GAGTACT46400.017.0903312
CATGGGG67550.015.583334
CATGGGA50000.015.3929064
TAGCCCT23100.015.2396144
Produced by FastQC (version 0.11.5)