FastQCFastQC Report
Mon 6 Feb 2017
SRR2077222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909852
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41310.1056561731748414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.038.5615231
GTATCAA101150.037.0426861
TATCAAC136200.027.0245322
ATCAACG134900.027.004463
TCAACGC134500.026.8801634
CAACGCA136800.026.4275555
AACGCAG141650.025.4012726
ACGCAGA163900.021.7884717
CGCAGAG167100.021.3042058
GAGTACT121150.020.92109112
AGAGTAC162450.020.76775611
CAGAGTA169800.020.15312410
GTACATG109750.019.9385281
TACATGG106300.019.9342062
AGTACTT128050.019.766913
GCAGAGT177000.019.6452279
GTACTTT131500.019.1759814
GTGGTAT20900.019.096431
ACATGGG107550.018.870923
TACTTTT146950.017.30910315
Produced by FastQC (version 0.11.5)