FastQCFastQC Report
Mon 6 Feb 2017
SRR2077221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301262
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5300.035.762821
TATAAGG400.002602974734.4738162
GTATCAA9800.030.945791
AGGTGCG450.00461192630.6739867
AAACCGT450.004634503730.6433950
TCTATAT658.6133514E-426.5536353
GTGGTAT2550.024.326371
TATCAAC12450.023.8132382
TAAGTAC902.1130795E-423.0131513
CCTATGG750.001983014423.0131513
CTAAGGG750.001984935323.0093214
CAGACCG750.001984935323.0093215
ATCAACG12950.022.6577363
TCAACGC13200.022.4863824
GAGTACT8500.022.31029512
AGTACTT8750.022.0705813
GTATAGC1103.2505734E-521.9597876
CAACGCA13600.021.8250185
ACTAAGC800.002884918621.574833
GTACTTT8800.021.553314
Produced by FastQC (version 0.11.5)