FastQCFastQC Report
Mon 6 Feb 2017
SRR2077221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301262
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.1048920872861496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG150.002363305568.93027537
TGGACCG200.00742217751.6289675
CGTGCGG451.8912979E-645.87716310
TCGTGCG451.8912979E-645.8771639
GGTATCA7500.044.071371
TTCGCAC400.002493291934.77766441
TATAAGG652.3532331E-531.7716732
GTATCAA13850.031.3232881
ACCGGGT450.004678115730.5847768
CTCGTAT1650.029.24314739
TCGCACC500.00752743127.74758142
CTATAGT500.00781477527.5354484
AGTTCGT500.00781477527.5354486
AGCGGCG500.00786562127.49888854
TCGTATG1950.026.75204840
TCATAGT658.761149E-426.4763952
TCTCGTA1607.8216544E-1125.8488538
GTGTAGC809.670005E-525.8230691
CGTATGC1900.025.6256541
TATGCCG1900.025.55698443
Produced by FastQC (version 0.11.5)