FastQCFastQC Report
Mon 6 Feb 2017
SRR2077220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3839135
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57150.031.362111
GGTATCA31050.030.416351
TCAACGC69250.025.5226554
ATCAACG70050.025.2821063
CAACGCA72050.024.5298355
TATCAAC72850.024.2739222
AACGCAG74200.023.7244746
ACGCAGA86000.020.268317
CGCAGAG87500.019.5665958
GAGTACT48800.019.15012412
AGAGTAC82850.018.8134311
GTACATG86900.018.7611521
TACATGG84700.018.3546142
ACATGGG86200.017.6217673
GTACTTT54900.016.8356514
CAGAGTA94600.016.75888810
GCAGAGT98750.016.4644859
AGTACTT57300.016.43100513
CGCGAGT1350.00323805315.33818824
GTATAGC13150.015.2026881
Produced by FastQC (version 0.11.5)