FastQCFastQC Report
Mon 6 Feb 2017
SRR2077220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3839135
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86800.035.3094861
GGTATCA43700.035.2638741
ATCAACG115500.026.0837843
TATCAAC115750.025.9687022
TCAACGC116400.025.6161964
CAACGCA119200.024.9852945
AACGCAG124050.023.8698236
ACGCAGA139150.020.992927
CGCAGAG139150.020.5614938
AGAGTAC134600.019.97546611
GTACATG84650.019.0987241
GAGTACT107450.019.07107212
CAGAGTA145250.018.79635210
TACATGG81600.018.5448072
AGTACTT112850.018.28036713
GCAGAGT152800.018.2061049
GTACTTT116650.017.48606314
ACATGGG83300.017.4640053
TACTTTT132000.015.54532415
ACTTTTT138100.015.46084216
Produced by FastQC (version 0.11.5)