FastQCFastQC Report
Mon 6 Feb 2017
SRR2077219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899970
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47600.034.1781581
GGTATCA23550.029.4183771
TCAACGC59100.027.1610724
ATCAACG60450.027.0146163
CAACGCA60450.026.440285
TATCAAC62250.026.0801542
AACGCAG62300.025.6515886
ACGCAGA70550.022.2116177
CGCAGAG71950.021.6857288
AGAGTAC69100.021.16088911
GTACATG75550.021.0775991
TACATGG74600.020.2378252
CAGAGTA78550.018.9542310
GCAGAGT80350.018.8184859
GAGTACT37600.018.80267312
ACATGGG80150.018.134793
AGTACTT45450.016.23943713
GTACTTT44800.015.70546214
GTGTAGC14300.015.4261471
CATGGGG55500.015.301134
Produced by FastQC (version 0.11.5)