FastQCFastQC Report
Mon 6 Feb 2017
SRR2077219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899970
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.039.031691
GTATCAA63250.035.937351
ATCAACG84600.026.6620563
TCAACGC84550.026.6371544
TATCAAC85900.026.4186692
CAACGCA85250.026.2974325
AACGCAG87750.025.2347436
ACGCAGA100550.021.6966197
CGCAGAG102050.021.0504918
GTACATG75550.021.0287111
AGAGTAC99050.020.64347311
GAGTACT72250.020.07219512
TACATGG75250.019.6024782
CAGAGTA107300.019.345510
AGTACTT78200.018.89652613
GCAGAGT113250.018.5731669
ACATGGG77550.018.533383
GTACTTT79750.018.40983414
TACACCG2701.6007107E-1017.8288945
TACTTTT89100.016.6225415
Produced by FastQC (version 0.11.5)