FastQCFastQC Report
Mon 6 Feb 2017
SRR2077218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014807
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48850.032.0276871
GGTATCA25200.028.8546771
ATCAACG56650.026.7424073
TCAACGC57700.026.372414
CAACGCA58600.025.9667265
TATCAAC62600.024.5552062
AACGCAG62400.024.273016
TAGCGCG750.001992068523.0036014
ACGCAGA70850.021.085887
CGCAGAG73000.020.1838028
GTATAGC12450.018.8223511
GTACATG69300.018.3496341
AGAGTAC72100.018.32034911
GAGTACT47950.017.91006712
TACATGG69650.017.4677372
CGCGATC1402.0819141E-417.25248323
CAGAGTA79950.017.15682210
GTGTAGC22850.016.8914181
GCAGAGT83150.016.6837739
GTACTTT53700.015.92968714
Produced by FastQC (version 0.11.5)