FastQCFastQC Report
Mon 6 Feb 2017
SRR2077218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014807
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.038.2009661
GTATCAA77050.034.2873571
ATCAACG98100.026.2946433
TCAACGC98600.026.0217764
TATCAAC100450.025.9194872
CAACGCA99550.025.7389035
AACGCAG103800.024.4199736
ACGCAGA120050.020.8371497
CGCAGAG121400.020.252878
AGAGTAC124100.019.14553811
GTACATG68400.019.060251
GAGTACT99200.018.44001612
CAGAGTA130800.018.4021210
GGACCGT4750.018.1017886
GCAGAGT134800.017.9075269
AGTACTT104850.017.5447313
TACATGG69150.017.5077742
GTACTTT107900.016.9920514
ACATGGG71800.016.382373
GTGTAGC21700.016.0105821
Produced by FastQC (version 0.11.5)