FastQCFastQC Report
Mon 6 Feb 2017
SRR2077217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5155014
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76150.032.7241021
GGTATCA36300.031.9030341
TCAACGC89150.027.142264
CAACGCA90900.026.6956655
ATCAACG91700.026.3900533
TATCAAC96000.025.6712342
AACGCAG97600.024.8243276
ACGCAGA118400.020.3754527
CGCAGAG119050.019.9760518
GTACATG122850.018.7693941
TACATGG122150.018.0874162
AGAGTAC115950.017.96080611
GAGTACT62200.017.57263812
CAGAGTA127700.016.97478310
GCAGAGT132300.016.5416479
ACATGGG128650.016.341773
AGTACTT73400.014.98666113
TAGCCCT30450.014.9635954
GTACTTT72100.014.82672214
GTATTAT21350.014.6907111
Produced by FastQC (version 0.11.5)