FastQCFastQC Report
Mon 6 Feb 2017
SRR2077217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5155014
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.040.3639261
GTATCAA118000.037.206041
ATCAACG156550.027.6223953
TCAACGC155850.027.5260734
TATCAAC158300.027.3387242
CAACGCA159450.026.7748365
AACGCAG165050.025.741536
ACGCAGA191700.022.040487
CGCAGAG194550.021.501828
GAGTACT142450.020.51519412
AGAGTAC191300.020.33871311
CAGAGTA205200.019.17948210
GTACATG125450.019.0865171
GCAGAGT209650.019.0190149
AGTACTT154500.019.00386413
GTACTTT158300.018.5337214
TACATGG126750.018.0745092
ACATGGG130850.016.6419493
TACTTTT180250.016.53059815
ACTTTTT188300.016.45203216
Produced by FastQC (version 0.11.5)