FastQCFastQC Report
Mon 6 Feb 2017
SRR2077216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977283
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62050.031.9366071
ATCAACG69300.027.4006943
TCAACGC69950.027.291124
CAACGCA72850.026.441125
GGTATCA35850.025.6675761
TATCAAC76750.024.879532
AACGCAG77150.024.8759176
ACGCAGA89900.020.9255587
CGCGTAC1004.2811717E-420.74020662
CGCAGAG92750.020.172548
GTACATG100700.018.6521871
AGAGTAC94200.018.52132411
TACATGG98750.018.289632
GAGTACT54450.017.73079512
CAGAGTA102350.017.34097110
GTGTAGC26300.017.1663631
GCAGAGT106150.017.0406659
ACATGGG102950.016.9354653
CGATCGT2251.109529E-716.86693242
GTACTTT62500.015.55975214
Produced by FastQC (version 0.11.5)