FastQCFastQC Report
Mon 6 Feb 2017
SRR2077216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977283
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96350.036.9219471
GGTATCA46800.035.874821
ATCAACG127150.027.214563
TCAACGC126800.027.1540434
TATCAAC128550.026.8646622
CAACGCA131250.026.1542475
AACGCAG135850.024.8382116
ACGCAGA155400.021.163937
CGCAGAG156150.020.9013358
AGAGTAC155850.019.96680811
GAGTACT125550.019.0891812
CAGAGTA163450.019.08277910
GTACATG93600.018.562281
GCAGAGT170450.018.521589
TACATGG91300.018.0461462
AGTACTT136150.017.98181713
GTACTTT137800.017.64706214
ACATGGG95650.016.9017853
GTATAGC15350.016.8100381
ACTTTTT165200.015.33410316
Produced by FastQC (version 0.11.5)