FastQCFastQC Report
Mon 6 Feb 2017
SRR2077215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4568914
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60750.032.451821
GGTATCA33050.029.095251
ATCAACG70600.026.797473
TCAACGC70900.026.633054
CAACGCA73550.025.767055
TATCAAC76950.024.9925272
AACGCAG77950.024.221636
ACGCAGA89050.020.8916477
CGCAGAG91150.020.0717268
GTACATG109200.019.6316641
TACATGG105200.019.1984632
AGAGTAC90150.018.85793911
ACATGGG106050.018.0024833
CAGAGTA98600.017.23048410
GAGTACT47600.016.37266212
GCAGAGT106450.016.084639
GTGTAGC22200.015.5251691
TATACTG18350.015.4259825
TCTATAC13350.014.9990823
CATGGGG69900.014.8157584
Produced by FastQC (version 0.11.5)