FastQCFastQC Report
Mon 6 Feb 2017
SRR2077215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4568914
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47800.039.372381
GTATCAA95750.034.6034771
ATCAACG127400.025.5689473
TCAACGC127550.025.4040364
TATCAAC130400.025.192012
CAACGCA129350.024.9707435
AACGCAG135600.023.769076
ACGCAGA154150.020.6010657
CGCAGAG155950.020.1354148
GAGTACT115600.019.48312812
GTACATG107650.019.4641511
AGAGTAC153800.019.33904511
TACATGG105500.018.8783112
AGTACTT121900.018.56083313
CAGAGTA162650.018.4577310
GTACTTT125350.018.0567114
ACATGGG105500.017.8999633
GCAGAGT172250.017.6496019
GTGGTAT24500.015.8688181
TACTTTT148400.015.35569115
Produced by FastQC (version 0.11.5)