FastQCFastQC Report
Mon 6 Feb 2017
SRR2077214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4052528
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49400.032.4396551
GGTATCA25700.028.6966291
ATCAACG57550.026.9971163
TCAACGC58900.026.4945934
CAACGCA61200.025.329645
TATCAAC63350.024.5914972
AACGCAG63500.024.1379476
ACGCAGA71150.021.203287
CGCAGAG73250.020.361528
GTACATG72950.019.038381
TACATGG71350.018.4045052
AGAGTAC75000.017.93105111
GAGTACT49400.017.52060912
ACATGGG74250.017.4375323
TAGCCCT27000.017.2625164
GCAGAGT84000.016.6466149
GGACCGT3750.016.5703756
GTGTAGC22050.016.5671391
GTACTTT55100.016.39815714
CAGAGTA83550.016.25294910
Produced by FastQC (version 0.11.5)