FastQCFastQC Report
Mon 6 Feb 2017
SRR2077214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4052528
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.037.3439031
GTATCAA73800.037.2554171
ATCAACG97550.027.7925363
TCAACGC98100.027.4613574
TATCAAC99200.027.3996282
CAACGCA100300.026.7211445
AACGCAG103200.025.8035686
ACGCAGA118200.022.1608287
CGCAGAG121150.021.5504538
AGAGTAC124650.019.89350511
GAGTACT97250.019.76926412
GTATCGA1952.2231688E-819.411411
CAGAGTA131000.019.35022210
GCAGAGT133900.019.034599
AGTACTT106250.018.2889113
CCGTACG950.007975602518.1030719
GTACTTT108100.017.91870314
GTACATG78800.017.6859261
TACATGG76400.016.7524682
TCGCGTC4600.016.44896319
Produced by FastQC (version 0.11.5)