FastQCFastQC Report
Mon 6 Feb 2017
SRR2077213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053404
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36000.030.4385811
GGTATCA19350.026.3560641
ATCAACG41950.025.2604033
TCAACGC42450.024.7973964
CAACGCA44700.023.6264135
TATCAAC46950.022.899122
AACGCAG45800.022.756056
GTACATG63250.019.88531
TACATGG62100.019.3657192
ACGCAGA54250.019.0210277
ACATGGG64550.018.3413683
CGCAGAG56800.018.1078198
AGAGTAC54850.016.46789711
GTATTAC7050.016.1296391
GTGTAGC16900.016.1079481
TACGACT2051.4391424E-515.152094
CATGGGG48300.015.1486054
TAGCCCT17400.014.8763344
CAGAGTA62200.014.79203210
GCAGAGT64450.014.6204029
Produced by FastQC (version 0.11.5)