FastQCFastQC Report
Mon 6 Feb 2017
SRR2077213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053404
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54000.034.881181
GGTATCA26950.033.2209631
ATCAACG67050.027.6763783
TCAACGC66650.027.4808864
TATCAAC68750.027.0926172
CAACGCA69950.026.2828675
AACGCAG71450.025.5865366
ACGCAGA84650.021.3358487
CGCAGAG86500.020.490898
AGAGTAC85200.019.95295711
GAGTACT65750.019.5223912
GTACATG54800.018.348471
CAGAGTA94600.018.22615210
GCAGAGT97100.017.9695479
TACATGG54300.017.7535252
GTACTTT71850.017.7259214
AGTACTT75000.017.02258113
TAGCCCT14950.016.1204094
ACATGGG56500.016.0869883
GTGTAGC15250.015.5803361
Produced by FastQC (version 0.11.5)