FastQCFastQC Report
Mon 6 Feb 2017
SRR2077212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425190
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36400.032.4717331
TCAACGC42500.026.9676154
ATCAACG42700.026.6812633
GGTATCA19700.026.0635031
CAACGCA43950.025.8417195
TATCAAC45700.025.18872
AACGCAG46950.024.0439366
ACGCAGA55700.019.956127
CGCAGAG56300.019.4985878
GTACATG55900.019.171731
TACATGG55650.019.0129152
AGAGTAC56350.017.7457711
ACATGGG58000.017.3214933
TATACCG1402.0740711E-417.2605425
CCGATCG1602.9376033E-517.2601859
CAGAGTA60350.017.01682710
GCAGAGT61400.016.9228529
GAGTACT32550.016.63180512
GTATAGC8450.015.9045231
CATGGGG36500.015.2274234
Produced by FastQC (version 0.11.5)