FastQCFastQC Report
Mon 6 Feb 2017
SRR2077212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425190
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.037.085931
GTATCAA51500.033.932821
ATCAACG64950.026.0515063
TATCAAC65350.025.9978392
TCAACGC64750.025.9726334
CAACGCA65600.025.3210225
AACGCAG68700.023.9281486
ACGCAGA81550.019.930847
CGCTATC2950.019.92807833
CGCAGAG81650.019.8373998
AGAGTAC79800.019.432211
GTACATG55550.018.641
GAGTACT59900.018.36840212
CAGAGTA86000.018.35187310
ACGCTAT3250.018.2148632
TACATGG55600.017.7526422
GCAGAGT89300.017.7136659
AGTACTT64650.017.44430213
TACGCTA3750.016.85753831
GTGTAGC11850.016.8373261
Produced by FastQC (version 0.11.5)