FastQCFastQC Report
Mon 6 Feb 2017
SRR2077211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980850
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56600.028.193231
ATCAACG63400.024.8876293
TCAACGC62650.024.7994754
CAACGCA63450.024.3776575
GGTATCA34350.023.5787831
AACGCAG66600.023.2732776
TATCAAC67000.023.2525842
ACGCAGA74650.020.4401157
CGCAGAG76200.019.7540058
AGAGTAC72250.019.0899411
GAGTACT45900.018.63036512
GTGTAGC23000.018.5812171
GTACATG75300.017.942021
GTATAGC16000.017.87881
TACATGG72300.017.6388822
ACATGGG72750.016.8481393
TAGCCCT28950.016.8159584
CAGAGTA84050.016.8118510
GCAGAGT89600.016.3375539
GTACTTT53400.016.2086814
Produced by FastQC (version 0.11.5)