FastQCFastQC Report
Mon 6 Feb 2017
SRR2077211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980850
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75400.032.9321371
GGTATCA39050.030.9129281
ATCAACG95050.025.28893
TCAACGC94500.025.1082654
CAACGCA96100.024.5110075
TATCAAC99450.024.4120792
AACGCAG100650.023.2321346
ACGCAGA115150.020.0496227
CGCAGAG115300.019.6512418
AGAGTAC113050.018.88356411
GAGTACT89900.018.46929612
CAGAGTA123900.017.48028410
AGTACTT97900.017.31119713
GTACTTT98050.017.15461214
GTACATG70500.017.073911
GCAGAGT130050.016.8930479
CGTGCGC6750.016.29766810
TACATGG71300.016.060522
TCGCGTC3900.015.87530317
GTGTAGG14200.015.5004981
Produced by FastQC (version 0.11.5)