FastQCFastQC Report
Mon 6 Feb 2017
SRR2077210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143589
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53850.030.3996071
GGTATCA29250.027.2173711
TCAACGC59750.025.7693164
ATCAACG60300.025.7657763
CAACGCA62900.024.752935
AACGCAG65350.023.5568056
TATCAAC67100.023.1151812
ACGCAGA77650.019.425277
CGCAGAG77600.019.263138
GTACATG79200.018.3631571
TACATGG76100.017.980972
AGAGTAC78300.017.79292911
CAGAGTA85850.016.70027410
ACATGGG77550.016.4282973
GAGTACT50850.016.2085912
GCAGAGT91400.015.9016379
CCTATAC9550.015.54583
GTATAGG9150.015.4426851
TAGCCCT25650.015.2088724
GTGTAGC25550.015.1073111
Produced by FastQC (version 0.11.5)