FastQCFastQC Report
Mon 6 Feb 2017
SRR2077210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143589
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.034.9752581
GTATCAA81800.034.7016531
ATCAACG105050.026.196533
TCAACGC106150.025.665814
TATCAAC109150.025.3700872
CAACGCA109050.024.8252495
AACGCAG112450.023.8605086
ACGCAGA130100.020.3183967
CGCAGAG130600.020.0672638
AGAGTAC130450.019.0320511
GAGTACT104250.018.60353512
GCAGAGT142700.017.906469
CAGAGTA142000.017.72789410
AGTACTT111250.017.52570313
GTACATG81150.017.2990071
GTACTTT117050.016.69351214
TACATGG79600.016.5082152
GTATAGC12600.016.3843881
ACATGGG80650.016.1226793
GTATAGG9200.015.7076411
Produced by FastQC (version 0.11.5)