FastQCFastQC Report
Mon 6 Feb 2017
SRR2077209_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077209_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193142
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC200.00736806851.7175112
CGACTAG200.00736806851.7175150
CTCAACG200.00737563151.7040942
GGTATCA4350.047.5439951
TTGCGGA306.2374567E-446.0906869
ATGAACG306.2775495E-446.0308234
GTGGTAT1000.041.3632771
CGCATCG350.001344034139.43450521
GTATCAA9050.033.8980831
ATAAGTA450.00459230230.6951873
TGGTATC1452.1827873E-1128.52642
TCGCCAG500.007684251827.6256725
ATATAAG500.007752722627.5755181
TCGTTTA500.00776254327.5683738
TATCAAC11850.026.179292
TCAACGC12050.024.6389054
ATCAACG12000.024.4602283
CAACGCA12450.023.8472945
AACGCAG12450.023.8472946
GTACATA953.071205E-421.7701471
Produced by FastQC (version 0.11.5)