FastQCFastQC Report
Mon 6 Feb 2017
SRR2077209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193142
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.1563616406581686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.11701235360511955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4900.053.2600141
GTATCAA10150.036.8760381
TGGTATC1154.9112714E-1132.8457072
CTAGCCC553.3375277E-431.2169934
TATCAAC13400.027.675962
GTGGTAT1457.3305273E-1026.0500451
TCAACGC14000.025.9992984
ATCAACG14350.025.8437543
CAACGCA14200.025.3847055
GTACATA700.001366639924.5276371
TAGGACA700.001366639924.5276374
AACGCAG14300.024.4869826
AGTGTCT750.002038856722.8984018
TATGCAG750.00204501822.8865285
ATGCAGC902.1895752E-422.8865286
GATCTCA953.1455763E-421.6932219
ATCTCAT800.002965996321.46724920
GAGGATA800.0029749421.4561186
ACGCAGA16350.021.405657
CGCAGAG16300.020.8614278
Produced by FastQC (version 0.11.5)