FastQCFastQC Report
Mon 6 Feb 2017
SRR2077208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893633
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64150.035.0283851
GGTATCA32300.033.2905731
ATCAACG79900.027.8203243
TCAACGC79450.027.672724
CAACGCA82350.026.7815
TATCAAC84050.026.7783472
AACGCAG85150.025.8169636
ACGCAGA98500.021.827697
CGCAGAG100450.020.8545278
AGAGTAC94950.020.1532911
GTACATG108650.020.0473291
TACATGG107450.019.5031812
GAGTACT49750.018.57332412
CAGAGTA105250.018.43291710
ACATGGG110800.018.0057453
GCAGAGT111400.017.8447329
GTACTTT55300.016.46214
CATGGGG69650.016.3530644
AGTACTT57950.016.06602913
GTATAGC12300.014.5702641
Produced by FastQC (version 0.11.5)