FastQCFastQC Report
Mon 6 Feb 2017
SRR2077208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893633
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45900.041.9745671
GTATCAA100200.037.2540051
ATCAACG138300.026.8358423
TCAACGC137700.026.7279624
TATCAAC139500.026.703972
CAACGCA142300.025.742765
AACGCAG146350.024.7953456
ACGCAGA168550.021.2268897
CGCAGAG172050.020.6893028
GAGTACT124750.020.0402812
AGAGTAC167700.019.8496211
GTACATG104850.019.1954541
CAGAGTA176450.019.02218610
AGTACTT132100.019.00334213
TACATGG104150.018.692992
GCAGAGT184250.018.6088669
GTACTTT135550.018.47724314
GTGGTAT18900.018.2032591
ACATGGG107650.017.1583793
TACTTTT151150.016.64864315
Produced by FastQC (version 0.11.5)