FastQCFastQC Report
Mon 6 Feb 2017
SRR2077207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838007
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49400.032.651291
GGTATCA25300.029.2886541
TCAACGC54200.028.7865454
ATCAACG55150.028.4806793
CAACGCA56250.027.860535
TATCAAC60000.026.480462
AACGCAG59750.026.2247666
ACGCAGA70850.021.776017
CGCAGAG71750.021.1675158
TCGCGCG900.00569806219.18916169
AGAGTAC73000.019.08263211
GTACATG77300.018.8154411
TACATGG76000.018.3661142
CAGAGTA78050.018.14748410
GTGTAGC23550.018.147311
GTATAGC16300.017.972681
GAGTACT45700.017.50480512
ACATGGG76200.017.4870593
TAGCCCT26950.016.9069614
GCAGAGT85150.016.8654849
Produced by FastQC (version 0.11.5)