FastQCFastQC Report
Mon 6 Feb 2017
SRR2077207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838007
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.039.562871
GTATCAA73200.035.103141
TCAACGC92100.026.8105054
ATCAACG93350.026.7462223
TATCAAC95050.026.3764062
CAACGCA93700.026.2789465
AACGCAG97050.025.4072786
ACGCAGA114500.021.1306517
CGCAGAG114350.020.7497778
AGAGTAC112950.019.66405911
GTACATG72650.019.2232531
GAGTACT88350.018.64066912
CAGAGTA120750.018.59387610
TACATGG73850.018.4410172
GCAGAGT126500.018.075579
AGTACTT95900.017.42409513
ACATGGG77250.017.1396683
TACACCG3850.016.9717795
GTACTTT98700.016.93774614
CGTGCGC5200.015.869081510
Produced by FastQC (version 0.11.5)