FastQCFastQC Report
Mon 6 Feb 2017
SRR2077206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382535
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43000.032.9441341
GGTATCA22050.031.1063611
TCAACGC50550.027.0425574
ATCAACG51750.026.4849363
CAACGCA52850.025.8660665
TATCAAC53900.025.4521662
AACGCAG56350.024.1337366
ACGCAGA65250.020.841937
CGCAGAG65350.020.5468648
GTACATG68450.018.4798031
AGAGTAC64550.018.32105611
TACATGG68750.017.497792
GAGTACT36950.017.35631412
GTGTAGC18000.017.2335471
CAGAGTA72000.016.99193210
ACATGGG69500.016.6409783
GCAGAGT78350.016.0801099
CCTATAC7950.015.6334263
TAGCCCT21100.015.05147654
GTACTAG7300.014.6367131
Produced by FastQC (version 0.11.5)