FastQCFastQC Report
Mon 6 Feb 2017
SRR2077206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382535
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.038.294051
GTATCAA69050.035.572321
ATCAACG87950.027.0219693
TCAACGC87600.026.9332284
TATCAAC90050.026.6591632
CAACGCA89400.026.5055755
AACGCAG93050.025.465866
ACGCAGA108000.021.6692967
CGCAGAG108700.021.324538
AGAGTAC109050.019.77032711
GAGTACT82900.019.41168812
CAGAGTA115300.019.147410
GCAGAGT121650.018.402619
GTACATG70550.018.236951
AGTACTT89500.018.09546913
GTACTTT92750.017.3962614
TACATGG67900.017.323322
GTGGTAT17500.016.316151
ACATGGG71150.015.9036143
TATGCCG4000.015.61405143
Produced by FastQC (version 0.11.5)