FastQCFastQC Report
Mon 6 Feb 2017
SRR2077205_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077205_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553301
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.033.090181
GTATCAA20100.032.0681151
TCAACGC24900.024.8132554
ATCAACG25500.024.367923
CAACGCA25600.023.8643365
AACGCAG26450.022.9654546
TATCAAC27350.022.811252
GTGTACG1451.2751356E-519.0173441
ACGCAGA31300.018.8555767
CGCAGAG32450.018.0815758
GTACATG31650.017.4250531
AGAGTAC31550.016.82965511
TACATGG31700.016.7451632
GTGTAGC7750.016.0113751
ACATGGG31800.015.74080853
CAGAGTA34400.015.72994110
ACACCGA2705.2261385E-815.3392986
ACCGGTG1350.00324716915.3308941
GCAGAGT36850.014.9874099
GTATAGG3001.19252945E-814.9365391
Produced by FastQC (version 0.11.5)