FastQCFastQC Report
Mon 6 Feb 2017
SRR2077205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553301
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.040.2957341
GTATCAA33000.035.0625231
TATCAAC42050.027.0215172
ATCAACG41500.026.964793
TATGCCG1705.456968E-1226.52270543
TCAACGC42050.026.366454
CAACGCA43550.025.3784265
AACGCAG45150.024.2503016
CTCGTAT1902.7284841E-1123.55947139
CGCTATC1605.6423232E-821.59097333
ACGCAGA50800.021.0576887
CGCAGAG51200.020.8387458
GTGGTAT7800.020.7501411
TATCTCG1851.2032615E-820.47437936
CGCTACG2252.1827873E-1020.1776328
AGAGTAC51000.019.97309711
TCGTATG2504.5474735E-1119.45183640
CAGAGTA53250.019.38828810
GAGTACT38350.019.11340512
GCCGTCT2608.185452E-1118.6757546
Produced by FastQC (version 0.11.5)