FastQCFastQC Report
Mon 6 Feb 2017
SRR2077204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659526
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.037.661871
GTATCAA57400.034.0427441
TCAACGC68800.027.4466654
CAACGCA71400.026.3984955
ATCAACG71750.026.2244833
TATCAAC73650.025.92652
AACGCAG73700.025.4327496
ACGCAGA84850.021.6835487
CGCAGAG86850.020.9869278
GAGTACT47950.019.99350212
CCGATCG1409.304342E-619.7260789
AGAGTAC87950.019.1734311
GTATACG2255.6188583E-918.3802811
CAGAGTA94600.018.2882410
GTACTTT53600.018.14581514
GCAGAGT97350.017.9074699
AGTACTT55700.017.8322313
GTACATG90350.017.8132081
TACATGG88500.017.525732
TTACGCG1402.0601321E-417.27617669
Produced by FastQC (version 0.11.5)