FastQCFastQC Report
Mon 6 Feb 2017
SRR2077204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659526
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.039.5541731
GTATCAA80650.037.787641
TCAACGC107700.027.3041864
ATCAACG109050.027.2184853
TATCAAC110800.026.9127542
CAACGCA111150.026.3016095
AACGCAG115000.025.3612636
ACGCAGA132650.021.630477
CGCAGAG135600.020.8395798
ATCGCGA850.004061703620.3457234
GAGTACT95450.020.1653512
GTACATG90550.019.9810221
TACATGG87900.019.6814882
AGAGTAC132700.019.6591411
CAGAGTA139400.018.96212210
AGTACTT101950.018.87967313
GCAGAGT144750.018.5717629
GTGGTAT16600.018.2345471
GTACTTT105950.018.17658814
ACATGGG91250.017.7904913
Produced by FastQC (version 0.11.5)