FastQCFastQC Report
Mon 6 Feb 2017
SRR2077203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4338836
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73350.024.6712991
ATCAACG81950.021.2339953
TCAACGC82450.021.2274194
CAACGCA84750.020.73285
TATCAAC87600.020.1085032
AACGCAG88800.019.5505776
GGTATCA47700.019.2218931
AGAGTAC83650.018.01394511
GTACATG86000.017.5953921
GTGTAGC24000.017.0910431
ACGCAGA100650.017.0081637
CGCAGAG100750.016.8217498
TACATGG86200.016.7559742
GAGTACT55650.016.29610612
ACATGGG87350.015.8105593
GAGTACA60450.015.62381651
CAGAGTA109550.014.65973910
GTATTAA22500.014.0941271
TACACTG30750.014.0328145
TAGCCCT28600.014.0014124
Produced by FastQC (version 0.11.5)