FastQCFastQC Report
Mon 6 Feb 2017
SRR2077203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4338836
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101450.030.2639831
GGTATCA55900.025.7708591
TATCAAC122150.024.9331262
ATCAACG120700.024.8624233
TCAACGC120850.024.6040124
CAACGCA123850.023.8687085
AACGCAG127650.023.0235216
AGAGTAC127800.020.62389611
GAGTACT102500.020.41736212
ACGCAGA144650.020.1127227
CGCAGAG146700.019.7032188
AGTACTT111300.018.92611713
GTACCGT3500.018.6600156
GTACTTT117100.018.02511214
CAGAGTA153550.017.79297410
GTACATG77650.017.3568671
TGTACCG5000.017.1868555
GCAGAGT162850.017.1369929
GTATAGC17300.017.0914551
CTCGTAT5750.016.18121139
Produced by FastQC (version 0.11.5)