FastQCFastQC Report
Mon 6 Feb 2017
SRR2077202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193778
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44350.034.8148841
GGTATCA23200.029.4142481
TCAACGC54500.027.5529564
ATCAACG55050.027.3451063
CAACGCA56400.026.6243345
TATCAAC57600.026.213242
AACGCAG57950.025.7306756
ACGCAGA68300.021.2247567
CGCAGAG70150.020.5682248
AGAGTAC65800.020.17180411
AACGTCG1301.18390846E-418.5727322
GTACATG75950.018.514541
CAGAGTA75200.018.42247410
GAGTACT36900.017.5644512
TACATGG75300.017.396332
GCAGAGT79700.017.2810279
ACATGGG74950.016.6758273
GTATAGC9300.015.5649191
GTATTAG7350.015.4741621
GTACTTT44050.015.34149714
Produced by FastQC (version 0.11.5)