FastQCFastQC Report
Mon 6 Feb 2017
SRR2077202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193778
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42630.13347828183424146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39500.040.258351
GTATCAA87700.037.167341
ATCAACG114850.027.9563263
TATCAAC115650.027.9121592
TCAACGC115200.027.4232924
CAACGCA116800.026.9297775
AACGCAG121250.025.9130466
ACGCAGA139300.022.2922047
CGCAGAG141250.021.7312858
GAGTACT109650.021.08668912
AGAGTAC136950.020.9783911
AGTACTT115950.020.02999113
CAGAGTA147300.019.71642910
GTACTTT116800.019.56846614
GCAGAGT150900.019.5659189
CGTAACG1150.001138250217.93465256
GTACATG78500.017.889641
TACATGG75500.017.7768122
TACTTTT132700.017.33880815
ACTTTTT140550.017.30269616
Produced by FastQC (version 0.11.5)