FastQCFastQC Report
Mon 6 Feb 2017
SRR2077201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036684
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.037.579281
GTATCAA62050.036.051571
ATCAACG76850.028.840343
TCAACGC77100.028.5648844
TATCAAC79850.027.756942
CAACGCA80250.027.4857525
AACGCAG82900.026.4782856
ACGCAGA95800.022.623887
CGCAGAG96050.022.2075128
GTACATG90750.021.0798551
TACATGG89100.020.8132612
AGAGTAC94450.020.6988211
CAGAGTA101250.019.57142610
GCAGAGT101750.019.5393989
GAGTACT59400.019.44534712
ACATGGG93150.019.1238823
AGTACTT65250.018.23309513
GTGGTAT13350.017.5569441
GTACTTT69100.017.11768314
GTGTAGC13700.017.1084081
Produced by FastQC (version 0.11.5)