FastQCFastQC Report
Mon 6 Feb 2017
SRR2077201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036684
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41830.13774893930352977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37900.044.254311
GTATCAA89850.039.1702961
TATCAAC120550.029.0765912
ATCAACG120400.028.4848943
TCAACGC121900.027.993434
CAACGCA124550.027.1761975
AACGCAG127350.026.55176
GTGGTAT13400.026.1619661
ACGCAGA147100.022.5269897
CGCAGAG147650.022.3647828
AGAGTAC146500.021.62238711
GAGTACT109750.021.38092612
TGGTATC15050.021.0068972
CAGAGTA152200.020.9490910
GCAGAGT156400.020.4527599
AGTACTT117700.020.2286613
GTACTTT124350.019.2109914
GTACATG91450.019.2049051
GTATCGA1651.8978717E-618.7470761
TACATGG89500.018.5837292
Produced by FastQC (version 0.11.5)