FastQCFastQC Report
Mon 6 Feb 2017
SRR2077200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492118
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40850.034.1667671
GGTATCA21250.031.7861561
TCAACGC52850.025.8654544
CAACGCA53800.025.6012125
ATCAACG54350.025.0895233
TATCAAC55950.024.2702562
AACGCAG57250.023.8765136
ACGCAGA66950.020.1086987
CGCAGAG68600.019.8274548
GTACATG78200.019.4344711
TACATGG78800.018.5446172
AGAGTAC68900.018.11723111
ACATGGG81050.017.9743963
TAGGTCG1804.178073E-617.2599665
CAGAGTA75500.016.98191510
GCAGAGT78100.016.6202649
CATGGGG51050.016.0935234
GAGTACT37700.015.91559212
GTATAGC12750.015.4065561
GTGTAGC18700.014.9274041
Produced by FastQC (version 0.11.5)